[PDF] Candida albicans cell shaving uncovers new proteins involved in cell wall integrity, yeast to hypha transition, stress response and host-pathogen interaction. | Semantic Scholar (2024)

It is observed that C. albicans ecm33/ecm33 mutant RML2U displays the inability of protoplast to regenerate the cell wall, activation of the CWI pathway, hypersensitivity to temperature, osmotic and oxidative stresses and a shortened chronological lifespan.

This first analysis of C. albicans HEVs shows their relevance to pathogenesis and possible new diagnostics or treatments, as well as proteins related to intracellular protein transport and components of the ESCRT pathway related to exosome biogenesis.

The aim of the present study was to identify the cell wall-attached proteins of these two species with the use of cell surface shaving and a shotgun proteomic approach, and extend the current knowledge regarding the molecular basis of virulence ofThese two non-albicans Candida species.

The comparative in silico analysis of proteins required for cell wall biosynthesis and functions containing 187 proteins of 92 different yeasts was performed in order to assess which proteins were broadly conserved among yeasts and which were more species specific.

Several known proteins involved in cell wall maintenance and fungal pathogenesis were identified at the cell surface as were a number of atypical cell wall components—pyruvate decarboxylase, enolase and glyceraldehyde-3-phosphate dehydrogenase which are so-called ‘moonlighting’ proteins.

Results demonstrate that VdPLP regulates hyphal growth and conidial production and is involved in stabilizing the cell wall, thus mediating the pathogenicity of V. dahliae.

The passage of EVs through fungal cell walls suggests remarkable molecular diversity and, consequently, a potentially variable influence on the host antifungal response.

The data indicate that C. auris produces several unique proteins during its biofilm growth, which may assist in the skin-colonizing lifestyle of the fungus during its pathogenesis.

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Proteomic analysis of differentially regulated proteins involved in the morphological transition from the yeast to the hyphal state is entailed, finding that 6–9% of total cell wall‐protein‐enriched fraction was found to be resistant to DTT/SDS extraction.

This is, to the authors' knowledge, the first systematic, quantitative analysis of the changes in the wall proteome of C. albicans upon hyphal induction and proposes new wall-protein-derived candidates for vaccine development.

This work reports the establishment of a reference map of proteins secreted by regenerating protoplasts by means of two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) and their identification by mass spectrometry that will allow comparative analyses to be carried out on a selected collection of mutants affected in the cell wall.

RNA analyses showed that the HYR1 mRNA was induced specifically in response to hyphal development when morphogenesis was stimulated by serum addition and temperature elevation, increases in both culture pH and temperature, or N-acetylglucosamine addition.

Functional and network interaction analyses of the 56 differentially abundant proteins identified by both approaches rendered an integrated view of metabolic and cellular process reorganization during the yeast-to-hypha transition, proposing a model of metabolic reorganization.

Caecm33 mutant strains displayed an aberrant morphology, being more rounded and bigger than the wild-type, suggesting morphogenetic defects, and CaEcm33p is required for normal C. albicans yeast-to-hyphae transition in vitro.

The results, which include new identified cell wall proteins, and comparison of proteins in blastospore and mycelial walls, will help to elucidate the C. albicans cell wall architecture.

It is suggested that the CWP-chitin linkage is an important retention mechanism of CWPs in C. albicans mycelial forms and this approach is therefore a powerful tool for obtaining a comprehensive and integrated view of the cell wall proteome.

A substantial number of the covalently linked CWPs of C. albicans are actively involved in cell wall remodeling and expansion and in host-pathogen interactions.

The number of identified secretory proteins in the growth medium has been substantially extended, and growth conditions strongly affect the composition of the secretome, indicating that C. albicans tightly regulates its secretome.

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